Immunoassay reagents used a monoclonal antibody against tissue-type plasminogen activator derived from human normal cells

摘要

PURPOSE:To enable the manufacture of a specified monoclonal antibody, by using a tissue type plasminogen activator sampled from a calture of cells derived from a normal human tissue. CONSTITUTION:A monoclonal antibody having such specificity as give by the formula can be manufactured with respect to t-PA sampled from a culture of cells derived from a normal human tissue. The monoclonal antibody obtained has a nature of being unable to bond to a fibrinolysis activity developed part and a fibrin affinity part of t-PA. But this method provides a monoclonal antibody which is reactive to a different antigen determinant part. To achieve this, desired miscellaneous cells are cultivated in an appropriate calture which comprises a RPMI1640 or the like containing about 10% of bovine embryonic serum and then, the monoclonal antibody can be obtained from the supernatant of the culture. The refining of an antihuman tissue type plasminogen activator monoclonal antibody from abdominal dropsy is conducted in accordance with the ordinary recovery method of antibodys from serum. Thus, a very high-purity t-PA can be obtained at a high recovery rate.