Method of detection null of the disease which accompanies L fucose

摘要

PURPOSE:To detect the specific diseases which accompany the metabolic abnormality of an L-fucose quantity by measuring the free type L-fucose quantity in comparison a vital sample with a normal value. CONSTITUTION:A reaction is effected by making formulation as shown in table 1 and the absorbancy at 492nm is determined, then the determined value is designated as ODt in order to measure the free type L-fucose quantity. The reaction using water in place of 70 unit/mlFDH is separately effected in the above-mentioned reaction system and the absorbancy at 492nm is determined. The determined value is designated as ODb to make ODt-ODb=DELTAOD. The reaction is effected by using water and 1mM L-fucose in place of urine in the above-mentioned reaction system and the absorbancy at 492nm is measured in case of determining the free type L-fucose quantity from the value of DELTAOD. A graph taking the absorbancy at 492nm at the axis of ordinate and the L-fucose quantity at the axis of abscissa is formed and the L-fucose quantity in the urine is determined from the calibration value and the determined DELTAOD value. Since this method enables the detection of a stomach ulcer, gastric cancer, hepatocirhosis, and hepatoma, the rate of group medical examinations, etc., is improved.