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Method and work station for microinjection into cells, or aspiration from individual cells or aspiration of whole cells from cell culture

机译:显微注射到细胞中或从单个细胞中吸出或从细胞培养中吸出全细胞的方法和工作站

摘要

In this computer-assisted system, a pointer which can be moved in the image on the monitor (10) is superimposed on the television image of the cell culture and is positioned by the operator on the cells to be injected by relative movement of the pointer/image in the television image. The coordinates of the cells indicated in this way are stored by the computer (11) which subsequently automatically moves the capillary tip to, and inserts it into, the selected cells. In an advantageous embodiment, the computer makes the insertion movement parallel to the long axis of the capillary by a superimposed movement (x) of the microscope stage and of the drive for adjusting the height (z) of the capillary (4) on the micromanipulator (3).
机译:在该计算机辅助系统中,可以在监视器(10)上的图像中移动的指示器叠加在细胞培养物的电视图像上,并且通过指示器的相对移动,操作者将其定位在要注射的细胞上/ image在电视图像中。以这种方式指示的细胞的坐标由计算机(11)存储,计算机随后自动将毛细管尖端移动到选定的细胞并插入到选定的细胞中。在有利的实施例中,计算机通过显微镜载物台和驱动器的叠加运动(x)进行平行于毛细管长轴的插入运动,以调节微操纵器上毛细管(4)的高度(z)。 (3)。

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