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Method and work station for microinjection into cells, or aspiration from individual cells or aspiration of whole cells from cell culture
Method and work station for microinjection into cells, or aspiration from individual cells or aspiration of whole cells from cell culture
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机译:显微注射到细胞中或从单个细胞中吸出或从细胞培养中吸出全细胞的方法和工作站
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摘要
In this computer-assisted system, a pointer which can be moved in the image on the monitor (10) is superimposed on the television image of the cell culture and is positioned by the operator on the cells to be injected by relative movement of the pointer/image in the television image. The coordinates of the cells indicated in this way are stored by the computer (11) which subsequently automatically moves the capillary tip to, and inserts it into, the selected cells. In an advantageous embodiment, the computer makes the insertion movement parallel to the long axis of the capillary by a superimposed movement (x) of the microscope stage and of the drive for adjusting the height (z) of the capillary (4) on the micromanipulator (3).
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