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The composition of inibina and processes for the use of the same

机译:伊尼宾娜的成分及其使用方法

摘要

The present invention relates to a method of accelerating the onset of puberty in avians, especially ratites and psittaciformes, by administering a heterologous protein comprising inhibin, or a fragment thereof, and a carrier protein. The present invention also relates to a method of increasing egg production in animals, particularly birds, more particularly ratites, via the administration of the heterologous protein. This invention further relates to the heterologous protein and a method for producing the heterologous protein. The heterologous protein can be either inhibin conjugated to the carrier protein or inhibin fused to the carrier protein. The method of producing the fused heterologous protein comprises inserting double stranded cDNA encoding inhibin, or a fragment thereof, into a vector which encodes a carrier protein such as maltose binding protein or bovine serum albumin. After inserting the vector into an expression system, the fused heterologous protein is expressed by the system. After purifying the expressed heterologous protein, an effective amount of the protein is administered to an animal such that an immunological response occurs in the animal against the heterologous protein.
机译:本发明涉及通过给予包含抑制素或其片段和载体蛋白的异源蛋白来加速禽类,特别是平胸类和鹦鹉形类动物的青春期发作的方法。本发明还涉及通过施用异源蛋白质来增加动物,特别是鸟类,更特别是平胸鱼类的产蛋量的方法。本发明进一步涉及异源蛋白和生产该异源蛋白的方法。异源蛋白可以是与载体蛋白缀合的抑制素或与载体蛋白融合的抑制素。产生融合的异源蛋白的方法包括将编码抑制素的双链cDNA或其片段插入编码载体蛋白如麦芽糖结合蛋白或牛血清白蛋白的载体中。将载体插入表达系统后,融合异源蛋白由系统表达。纯化表达的异源蛋白质后,将有效量的蛋白质施用于动物,以使动物针对异源蛋白质发生免疫反应。

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