Isolated biologically active human lymphotoxin derivative and antibody neutralizing its cytolytic activity, nucleic acid encoding the lymphotoxin derivative, replicable vector comprising the nucleic acid, heterologous cell transformed with the nucleic acid, and method comprising preparing the lymphotoxin derivative.

摘要

Lymphotoxin (I) absolutely free from homologous proteins that are unidentified or present in unknown amounts is new. Unglycosylated (I) is new. Variant (I) in which an amino acid residue in the (I) sequence has been deleted or substd. by another residue, or in which another residue has been inserted, is new. Provided that (I) having a sequence with an amino terminal at Leu(+)1 or His(+)23 is excluded. Nucleic acid encoding (I) and selected from (a)-(c) is new. (a) DNA encoding (I) free from DNA encoding other proteins of the organism from which the DNA originated; (b) DNA encoding (I) free from untranslated intervening sequences; and (c) nucleic acid capable of hybridising to nucleic acid encoding (I), provided that the acid does not have the nucleotide sequence of natural DNA or RNA encoding (I).