首页>
外国专利>
Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase
Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase
展开▼
机译:编码脱乙酰氧基头孢菌素C合成酶和脱乙酰头孢菌素C合成酶的重组DNA表达载体和DNA化合物
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to DNA compounds and expression vectors that encode deacetoxycephalosporin C synthetase (DAOCS) and deacetylcephalosporin C synthetase (DACS) activities. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, and Cephalosporium. The invention also relates to the regulatory elements of the Cephalosporium acremonium DACS/DAOCS gene. A method for selecting Penicillium transformants, including those able to synthesize cephalosporin antibiotic due to the presence of the DACS/DAOCS-encoding DNA, is also provided. This transformation system utilizes an acetamidase gene for purposes of selecting transformants.
展开▼