首页> 外国专利> METHOD FOR PRODUCING DNA MOLECULES CODING FOR PECTIN LYASES AND/OR CONTAINING PROMOTER, SIGNAL AND TERMINATOR SEQUENCES THEREOF

METHOD FOR PRODUCING DNA MOLECULES CODING FOR PECTIN LYASES AND/OR CONTAINING PROMOTER, SIGNAL AND TERMINATOR SEQUENCES THEREOF

机译:生产用于果胶裂解酶和/或包含启动子,信号和终止子序列的DNA分子的方法

摘要

Recombinant DNA molecules coding for pectin lyase (PL) expression systems and derivatives thereof, such as the structural genes of PLA, PLB, PLC, PLE and PLF, and corresponding regulatory sequences, e.g. promoter, signal and terminator sequences, and hybrid vectors comprising corresponding DNAs, including hybrid vectors with DNA coding for homologous or heterologous polypeptides, hosts, especially filamentous fungi, e.g. Aspergillus hosts, transformed by said vectors, methods for the preparation of said recombinant DNA molecules and said hosts and the use of the recombinant DNA molecules for the preparation of new expression systems. A further objective is the preparation of polypeptides by means of said DNAs and said hosts.
机译:编码果胶裂解酶(PL)表达系统及其衍生物的重组DNA分子,例如PLA,PLB,PLC,PLE和PLF的结构基因,以及相应的调控序列,例如PLA,PLB,PL和PLF。启动子,信号和终止子序列,以及包含相应DNA的杂种载体,包括具有编码同源或异源多肽的DNA的杂种载体,宿主,尤其是丝状真菌,例如大肠杆菌。通过所述载体转化的曲霉宿主,所述重组DNA分子和所述宿主的制备方法以及重组DNA分子在制备新表达系统中的用途。另一个目的是借助于所述DNA和所述宿主制备多肽。

著录项

  • 公开/公告号HU212832B

    专利类型

  • 公开/公告日1996-12-30

    原文格式PDF

  • 申请/专利权人 CIBA-GEIGY AG.;

    申请/专利号HU19890003823

  • 发明设计人 HEIMJUTTA;MEYHACKBERND;VISSERJACOB;

    申请日1989-07-27

  • 分类号C12N15/60;C12N15/80;

  • 国家 HU

  • 入库时间 2022-08-22 03:26:58

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