METHOD TO INHIBIT THE CONVERSION OF HUMAN FACTOR X TO HUMAN FACTOR Xa BY MEANS OF A REAGENT AND PHARMACEUTICAL COMPOSITIONS CONTAINING SAID REAGENT

摘要

A method to inhibit the conversion of human Factor X to human Factor Xa, which method comprises contacting human Factor X with a reagent selected from the group consisting of: (i) a reagent capable of specifically binding sialic acid residues that are a(2-6) linked to galactose (SA(2-6)aGal) or N-acetylgalactosamine (SA(2-6)aGa1N-Ac) residues (said reagent abbreviated herein as SA/Ga1/Gal NAc binding reagent), in an amount and for a time sufficient to complex to Factor X; (ii) a reagent capable of cleaving glycosylation moieties from Factor X, in an amout and for a time sufficient to cleave said glycosylation moieties; (iii) a reagent capable of binding to the glycosylation moieties of the activation peptide of Factor X, in an amount and for a time sufficient to bind said peptide; and (iv) a soluble analog of SA/Ga1/Ga1NAc under conditions which would normally, in the absence of said soluble analog, effect the conversion of Factor X to Factor Xa.