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Spectrophotometric method for selenium determination

机译:分光光度法测定硒

摘要

The invention relates to biotechnology and may be applied for quantitative determination of the selenium within the organic substrata when receiving some of the albumens and other biological active substances, comprising selenium.The summary of the invention consists in the fact, that the proposed method for the selenium determination (IV) comprising acidic decomposition of the organic substratum, the leading of the pH mineral solution to 2,5-3,0, the separation of the selenium (IV) and its converting into the coloured complex combination with the application of the p-pa dithizone into CCl4 with the subsequent spectrophotometication of the solution, wherein pH mineral solution is stabilized by means of 0,1 M buffer solution citric acid - citric acid natrium, but the dithizone solution into CCl4 is stabilized by adding 0,2% ionol.The technical result of the invention consists in increasing the selenium separation degree from the present in the medium cations.
机译:本发明涉及生物技术,并且当接收一些包含硒的蛋白和其他生物活性物质时,可用于定量测定有机基质中的硒。发明内容因此,提出的用于硒的测定(IV)包括有机基质的酸性分解,pH矿物溶液的pH值为2,5-3,0,硒的分离(IV)以及在使用有机溶剂的情况下将其转化为有色络合物的方法对苯二硫酮成CCl4溶液,并随后进行分光光度化,其中pH矿物质溶液通过0.1 M柠檬酸-柠檬酸钠缓冲溶液稳定,而双硫th溶液进入CCl4的溶液中加入0.2%稳定本发明的技术结果在于增加与中等阳离子中存在的硒的分离度。

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