Fremgangsmåte for påvisning og/eller eventuelt kvantifisering og/eller separering av apoptotiske celler i eller fra en pröve

摘要

A procedure to measure apoptosis in physiology and pathology in order to support clinical diagnosis and monitor efficacies of drugs and therapies. In particular, a method for detecting and/or optionally quantifying and/or optionally isolating apoptotic cells in a sample, comprising: a) contacting the sample with a detectable reagent having high affinity for phosphatidyl serine and b) qualitatively and/or optionally quantitatively detecting cells that have reacted with the detectable reagent having high affinity for phosphatidyl serine preferably intact cells, said detection occurring before or after an optional step c) for isolating apoptotic cells from non-apoptotic cells, which isolation can occur due to the fact that apoptotic cells have been provided in step a) with a detectable reagent having high affinity for phosphatidyl serine, said reagent also being selectable in a manner known per se. A suitable reagent having high affinity for phosphatidyl serine is a polypeptide or protein classified as an annexin. A kit for carrying out such methods.