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Process for the determination of the proportion of a protein of merger with a mixture of protein and the product of proteolytic degradacao merger with
Process for the determination of the proportion of a protein of merger with a mixture of protein and the product of proteolytic degradacao merger with
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机译:测定蛋白质与蛋白质的混合物与蛋白水解降解兼并产物的混合物的比例的方法
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摘要
In the method, the mixture of fusion protein and degradation product is diluted with bovine serum albumin. One portion of the diluted mixture is mixed with alpha -amylase solution, incubated at 37 DEG C for /= 30 minutes, alpha -amylase reagent is added and the remaining activity in the alpha -amylase solution is measured by spectrophotometry. The other portion of the diluted mixture is mixed with alpha -amylase reagent and, after mixing, alpha -amylase solution is added and the enzyme kinetics (course of the reaction) are measured by spectrophotometry. All the measurements are compared with standards of fusion protein, tendamistat, degradation product and uninhibited alpha -amylase reaction, and the content of fusion protein in the mixture is determined from the comparison. IMAGE
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