DETERMINATION OF THE REACTIVITY OF LYMPHOCYTES TO SPECIFIC ANTIGENS IN THE BLOOD.

摘要

A SAMPLE OF BLOOD FROM A PATIENT IS INCUBATED WITH AN ANTIGEN AND TESTED FOR A RESPONSE OF LYMPHOCYTES, THAT IS, AN ACTIVATION OF LYMPHOCYTES AND / OR A CONVERSION OF LYMPHOCYTES TO LYMPHOBLASTES, WHICH INDICATES AN PREVIOUS EXPOSURE OF THE PATIENT TO THE ANTIGEN. A POSITIVE RESPONSE INDICATES THE PRESENCE OF PREVIOUS EXPOSURE TO PREVIOUS DISEASES OR CLINICAL CONDITIONS SUCH AS, FOR EXAMPLE, PARASITIC DISEASES, TUBERCULOSIS, GONORRHEA, INFECTIONS WITH FUNGI, RICHES, INFECTIONS, AND RICHES. THE TOTALITY OF THE PATIENT'S BLOOD IS INCUBATED WITH THE ANTIGEN OF THE DISEASE OR CONDITION FOR WHICH THE PATIENT IS BEING TESTED. AFTER AN APPROPRIATE TIME, A FLUORESCENT DYE OR DYE THAT HAS AN AFFINITY FOR A DISCRIMINATING CHARACTERISTIC OF ACTIVATED LYMPHOCYTES OR LYMPHOBLASTS, AS FOR EXAMPLE: INTRACELLULAR CALCIUM; SURFACE ACTIVATION ANTIGENS AS FOR EXAMPLE ASSIGNMENT RECEIVER; HLADR; LEU-23; AND SIMILAR. THE INCUBATED BLOOD IS THEN INTRODUCED WITHIN A TRANSPARENT TUBE CONTAINING A FLOAT THAT CONCENTRATES THE CONSTITUTIVE LAYERS OF THE CUSHIONING WRAP AFTER THE CENTRIFUGATED BLOOD SAMPLE. THE CONCENTRATED LYMPHOCYTES LAYER IS THEN EXAMINED TO SEE THE FLUORESCENCE OR COLORATION THAT IS INDICATIVE OF THE PRESENCE OF THE ACTIVATED LYMPHOCYTES OR LYMPHOBLASTS AND THEIR CONCENTRATION. FLUORESCENCE OR COLORATION CAN BE QUALIFIED AND / OR QUANTIFIED BY A READING INSTRUMENT.