Preparation and use of synthetic human antibody libraries ( 'synthetic human antibody libraries').

摘要

The invention relates to the production and application genebank HUMAN SYNTHETIC ANTIBODY (Huak) or parts ANTIBODY CONTAINING ANTIGENS SPHERES. STARTING FROM FRAMES ON A TABLE Huak Incidentally, they ARE FORMED hypervariable CDNA SYNTHETIC ANTIBODY AREAS THROUGH Oligonucleotide approximate "casual" ( "RANDOM"). THE AMINOACIDOS RELATIVELY PRESERVED IN AREAS hypervariable ARE CONSIDERED BY THE SELECTION OF RELEVANT nucleotide during the synthesis of oligonucleotide, and the relationship of the nucleotide SET FORTH BETWEEN THEM IS GIVEN SO THAT A "NONSENSE-CODON" One can only hope maximum 89. IN EACH POSITION BY THESE Huak cDNA EXPRESSION OF SYNTHETIC MICROBICAS expression systems, such as the VECTOR PFMT described below in E. coli, pUTS A library ASI A Huak SYNTHETIC WITH AN EXTENSIVE INVENTORY FOR REVIEW ( "SCREENEN ") SELECTED IN VITRO antigen.