LINKER INSERTION MUTAGENESIS BY MEANS OF THE TRANSPOSITION SYSTEM OF INSERTION SEQUENCE IS21

摘要

A genetic engineering process allows 4 to 11 codons to be inserted into any site of cloned genes. In genes that code for proteins, the reading pattern is not modified by an insertion. The insertions modify the protein structure, so that the proteins may be given a modified biologic activity. Transposition may be carried out according to this process both in vivo, for example in Escherichia coli, or in vitro. The process may also be used to insert epitopes.