METHOD FOR EX VIVO PROLIFERATION AND DIFFERENTIATION OF ADULT PANCREATIC ISLET CELLS, MEDIA USEFUL THEREFOR AND USES THEREOF

摘要

A method for inducing the profileration and differentiation of neonatal and/or adult human or non-human pancreatic islets to produce a product useful, for exmple, as a therapeutic agent for treatment of diabetes has been developed. Invention method involves a series of complex cell culture media containing necessary nutrients and growth factors, a human cytokine (hepatocyte growth factor or scatter factor), a microgravity culture vessel for promoting three dimensional growth, and molecular biology assays for measuring insulin promoter activity. A method for providing a hybrid organoid comprising a combination of donor and recipient cell types is also described.