The diagnostic method N that immune response cell-mediated caused by immune response and on the other hand vaccine inoculation cell-mediated caused by the on the one hand active tuberculosis as caused by the bacterium (mycobacterium tuberculosis, mycobacterium africanum and Mycobacterium bovis) for belonging to tuberculosis complex can be distinguished has treating tuberculosis immunogenicity. Additionally provide a kind of diagnostic kit, including polypeptide (such as, MPT64), the polypeptide can cause delayed allergy (Dth) in active tuberculosis animal, but delayed allergy cannot be caused being inoculated in animal (for example, BCG plants of mycobacterium tuberculosis var bovis: Denmark 1331) lungy with immunogene agent. It additionally provides the polypeptide fragment of the t cell epitope comprising MPT64 and encodes the nucleic acid fragment of these polypeptide fragments.
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