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COVALENT DIMERS OF KIT LIGAND AND FLT-3/FLK-2 LIGAND
COVALENT DIMERS OF KIT LIGAND AND FLT-3/FLK-2 LIGAND
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机译:试剂盒配体和FLT-3 / FLK-2配体的等效价
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摘要
A modified form of KL, the ligand for the c-Kit proto-oncogene, has been prepared wherein the protein is stabilized by an intermolecular covalent linkage. The protein can be prepared by expression of a recombinant protein which is dissolved in denaturant and refolded under conditions resulting in a disulfide linked dimer. Examples demonstrate the purification and characterization of this disulfide-linked cysteine dimer kit ligand (KL-CD) which contains at least one intermolecular disulfide bond and has at least ten-fold greater activity in promoting cell proliferation than native, non-covalently linked KL, as measured in in vitro assays.
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