Streptavadin and biotin-based optical solid-phase biosensor

摘要

Solid phase optical biosensor is claimed which has receptor biomolecules for the specific recognition of analytes utilising the Forster energy transfer (Resonant Energy Transfer; RET) between two fluorescent dyes F1 and F2. The biosensor comprises a transparent carrier supporting alternating polyanion and polycation layers. The uppermost layer contains a biotin-substd. polycation in which the degree of substitution is 20-80 (pref. 30-70, esp. 40-60) mole %, based on the number of equiv. cationic gps. The biotin-substd. polycation layer is covered with streptavidin bonded to it. There are additional biotin-substd. receptor biomolecules, pref. antibodies. The receptors bond with an analyte which is labelled with a fluorescent dye (F2). A fluorescent dye (F1) is bonded to the basic polyion layers, the streptavidin, the biotin-substd. receptor biomolecules or the antibodies.