EXPRESSION OF HETEROLOGOUS PROTEINS IN ATTENUATED BACTERIA USING THE HTRA-PROMOTERS IN BACTERIA DEFECTIVELY HAZARDOUS USING HTRA-PROMOTERS

摘要

The present invention provides a DNA construct comprising a DNA construct comprising an htrA promoter operably linked to a DHA sequence encoding one or more heterologous proteins, a replicable expression vector containing said construct, and a toxic attenuated bacterium containing said construct or vector /RTI The present invention also relates to a vaccine composition comprising a weakly toxic bacterial as defined above, or a fusion protein expressed from a construct as defined above, and a pharmaceutically acceptable carrier.