Method for culturing liquid spawn of basidiomycetes, its cultivation apparatus and liquid spawn

摘要

The present invention relates to a liquid spawn cultivation method of basidiomycetes, a cultivation apparatus thereof, and a liquid spawn according to the method, and in particular, a cultivation method and a cultivation apparatus capable of producing a large amount of liquid spawn useful for the cultivation of various mushrooms of high quality and a cultivation apparatus and the method It relates to a liquid spawn obtained by.;The present invention is a liquid culture method of ordinary basidiomycetes, the pre-culture step (a) 2 to 7% (w / v) carbon source, 0.1 to 1.0% (w / v) nitrogen source, KH ₂ PO _{4 0 to} 0.15 Inoculate the plated basidiomycete in the preculture consisting of% (w / v), MgSO ₄ 7H ₂ 0 0-0.15% (w / v), and the remaining distilled water, and (b) the inoculated preculture Incubated for 6 to 8 days in a shaker at 80 to 200 rpm and 22 to 30 ° C .; The above liquid culture process was carried out by (a) 2 to 7% (w / v) carbon source, 0.1 to 1.0% (w / v) nitrogen source, _{0 to} 0.15% (w / v) KH ₂ PO ₄ , MgSO ₄ 7H ₂ 0-0.15% (w / v), and a liquid culture medium consisting of the remaining distilled water, (b) aseptically inoculate the pre-inoculation source with the liquid culture solution, and (c) the inoculated above The liquid culture consists of aeration for 5 to 12 days at 5 to 100 rpm, the sterilized air at 0.1 to 1.0 vmv at 22 to 28 ° C.;The present invention is characterized in that after collecting the prokaryote of basidiomycetes, the pre-cultivation process and the liquid culture process consists of a series of inseparably connected series under strict conditions, respectively, and forcibly stirring the liquid culture. will be.