STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF SPECIFIC DNA-PROBE USED FOR DIFFERENTIATION OF TOXIGENIC AND NONTOXIGENIC CORYNEBACTERIUM DIPHTHERIAE AND COMPLEMENTARY TO TOX₉₉ GENE DETERMINING SYNTHESIS OF DIPHTHERIA TOXIN

摘要

FIELD: medicine, molecular biology. SUBSTANCE: method involves nucleic acid hybridization. Invention relates to preparing strain Escherichia coli C600 r^-m^- (pABC 124 tox AB') - a producer of specific DNA-probe for differentiation of toxigenic an nontoxigenic strains of Corynebacterium diphtheriae. Strain provides high yield of plasmid DNA pABC 124 tox AB' which is used for DNA-DNA hybridization as specific probe. EFFECT: preparing strain indicated above, high yield of plasmid DNA.