METHOD OF PREPARING ENZYME HYDROLYZATE FROM THE WEB-FOOTED MUSCLE TISSUE AND NUTRIENT MEDIUM 'CELAT' FOR EUCARYOTIC CELL CULTIVATION

摘要

FIELD: medicinal biotechnology, veterinary science. SUBSTANCE: method involves milling web-footed muscle tissue, mixing with distilled water at mass ratio = 1:2, hydrolysis in the presence of enzyme-containing web-footed organs at 40-42 C, at pH = 7.6-7.8 for 4-5 days in the presence of chloroform followed by heating biomass at 80-90 C for 5-10 min, precipitation of high-molecular compounds firstly in the presence of 20-22% calcium chloride solution and then - at protein isoelectric point, filtration and drying preparation. Nutrient medium "Celat" for eucaryotic cell cultivation has enzyme hydrolyzate of web-footed muscle tissue, cattle serum blood and Henx solution at the following ratio of components, vol.%: enzyme hydrolyzate of web-footed muscle tissue 0.15-0.20; cattle serum blood 5-10, and Henx solution - the rest. EFFECT: improved method of preparing. 2 cl, 4 tbl