DNA MOLECULE ENCODING ANALOG OF TRYPTOPHAN SYNTHASE BETA-SUBUNIT AND A METHOD OF INDOLE BIOSYNTHESIS

摘要

FIELD: molecular biology. SUBSTANCE: invention relates to DNA molecules encoding β-subunit of the modified tryprophan synthase. These polypeptide analogs at their expression in recombinant host-microorganism promote the producing and accumulation of significant levels of intracellular indole. Indole produced by this method in the presence of aromatic dioxygenase can be converted to indoxyl that is oxidized to indigo on air. EFFECT: improved method of indole biosynthesis.