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e - coli derived upstream located in yeast effective regulatory sequences

机译:大肠杆菌上游位于酵母中的有效调控序列

摘要

A hybrid upstream regulatory sequence including an upstream regulatory sequence of E. coli origin and yeast upstream regulatory sequence, which hybrid upstream regulatory sequence can function in a yeast cell; a hybrid promoter including the regulatory sequence of E. coli origin and a TATA region of a yeast promoter, which hybrid promoter can also function in a yeast cell; a hybrid promoter including the upstream regulatory sequence of E. coli origin and a yeast promoter, which hybrid promoter can function in a yeast cell; and a plasmid useful for a test of an upstream regulatory sequence, including a TATA region of a yeast promoter, a structural gene containing a translation start codon, and a yeast origin of replication.
机译:杂种上游调控序列,包括大肠杆菌来源的上游调控序列和酵母上游调控序列,该杂种上游调控序列可以在酵母细胞中起作用;杂种启动子,其包括大肠杆菌来源的调控序列和酵母启动子的TATA区,该杂种启动子也可在酵母细胞中起作用;杂种启动子,其包括大肠杆菌来源的上游调控序列和酵母启动子,该杂种启动子可在酵母细胞中起作用;以及用于测试上游调节序列的质粒,所述上游调节序列包括酵母启动子的TATA区,包含翻译起始密码子的结构基因和酵母复制起点。

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