A non-haemolytic protein, which may be obtained from Aeromonas sp., preferably A.salmonicida , having CIF activity, but lacking GCAF activity, is characterised by: (i) a molecular weight of ca . 30kDa.; (ii) a pI value of ca . 5.5; (iii) cytotoxic to ca . 100000 lymphocytes at levels of ca . 200ng.; (iv) cytotoxic to ca . 100000 AS2 tissue culture cells at levels of ca .200ng. The protein may be in vaccine formulation, especially for immunisation of fish against furunculosis.
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