Molecular cloning and expression of G-protein coupled receptors

摘要

The cloning and expression of two novel rat cDNAs "H218" and "rat-edg") which encode two members ("p.sup.H218 " and "p.sup.rat-edg ") of the G- protein coupled receptor superfamily of proteins is described. The amino acid sequence similarity between "p.sup.H218 " and "p.sup.rat- edg " suggests that they may be activated by the same endogenous ligand(s) . The expression pattern of mRNA transcripts of both genes in cell lines, various rat tissues and developing rat brain suggests that they both play a role in cell proliferation and/or differentiation. The polynucleotide molecules, proteins, and antibodies of the subject invention can be used in both diagnostic and therapeutic applications.