Genetic footprinting: insertional mutagenesis and genetic selection

摘要

Functional analysis of genes is provided by employing insertional mutagenesis to a population of DNA molecules containing the gene of interest. The DNA is subjected to a degree of insertion, where the total population of molecules provides for a substantially complete representation of insertion across the length of DNA. The DNA is then subjected to a functional selection. The effect of the selection is determined by amplifying DNA isolated from selected and control populations, and determining whether a particular region is over or underrepresented in the amplified DNA.