A hemoglobin-encapsulated liposome is provided. The hemoglobin- encapsulated liposome comprises a liposome and an enzymatically active hemoglobin solution encapsulated therein. The hemoglobin solution is supplemented with glucose; and at least one member selected from the group consisting of adenine, inosine, and adenosine triphosphate; and optionally, further with phosphoenol pyruvate. The thus supplemented hemoglobin solution encapsulated in the liposome exhibits an improved resistance to conversion to methemoglobin as well as an adequately regulated affinity for oxygen. Therefore, the hemoglobin-encapsulated liposome of the present invention is useful as a blood substitute.
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