Three-dimensional human cell cultures in heart valves on the framework and its use

摘要

(57) [Abstract] The present invention relates to a method of growing cells and tissues by a variety of three-dimensional culture using human fibroblasts in culture medium in in vitro. In accordance with the present invention, stromal cells (including cardiac fibroblasts and human skin, but are not limited to) are grown and inoculated into the framework on the three-dimensional scaffold or (scafold) a. Human fibroblasts are composed of leaflets organs and the aortic wall or a heart valve does not consist of cells is formed as a three-dimensional structure that secrete human matrix proteins, with a gap space which is bridged by the stromal cells , to replace it and compensate for the matrix of the pig. Stromal tissue so formed is a support, necessary for growth and maintenance of long-term survival of the cells in culture medium, and is required for the growth and survival of cultures implanted in vivo, and / or Na, and supplies the regulators and growth factors. When grown in this three-dimensional system, to form tissue constituents allogeneic cells grown matures, the corresponding in vivo can be separated properly.