REDIFFERENTIATION STOCK AND METHOD FOR GROWING NEW VARIETY STOCK

摘要

PROBLEM TO BE SOLVED: To obtain double flower stock having excellent quality by fertilizing non-branching stock with self-pollination, collecting immature seeds and redifferentiating these seeds under specific conditions. ;SOLUTION: The non-branching stock, such as KUROKAWA cherries, is fertilized by self-pollination and the immature seeds are collected. The tissue pieces taken out of these immature seeds are cultured in the basic medium added with a plant growth control agent, such as auxin, to derive calli. The double spread stock calli selected from the calli maintained and propagated in the basic medium added with this plant growth control agent are first transplanted to a high-concentration medium contg. a medium solidifying agent at a high concn., by which small shoot lumps are grown. These small shoot lumps are then transplanted from the high-concentration medium to the low- concentration medium of the low concn. of the medium solidifying agent and are cultured there, by which the small shoot lumps are redifferentiated to plant bodies. The tissue culture seedlings of the stock are thereby obtd. and the stock of the new variety different from the mother variety is grown.;COPYRIGHT: (C)1998,JPO