ENZYMATIC SYNTHESIS OF N-FORMYL-ALPHA-L-ASPARTYL-L-PHENYLALANINE METHYL ESTER

摘要

PROBLEM TO BE SOLVED: To efficiently obtain the subject compound useful for artificial sweetener, etc., by adding a water-immiscible solvent to an aqueous solution of a thermolysin-like metal protease and condensing a protected aspartic acid with a phenylalanine ester in an aqueous solution. ;SOLUTION: In this method for synthesizing N-formyl-α-L-aspartyl-L- phenylalanine methyl ester (F-APM) by an enzyme by a condensation reaction between N-formyl-L-aspartic acid and L-phenylalanine methyl ester or D,L- phenylalanine methyl ester, an organic phase comprising a water-immiscible solvent (e.g. tributyl phosphate) is supplied to an aqueous solution phase containing a thermolysin-like metal protease, the condensation reaction is advanced in the aqueous solution phase and the formed F-APM is transferred from the aqueous solution phase to the organic phase to efficiently give the objective compound useful as aspartame as an artificial sweetener having degree of sweetness of 200 times as much as sugar.;COPYRIGHT: (C)1998,JPO