PURPOSE: To improve the accuracy of high performance liquid chromatographic (HPLC) analysis by making the flow velocity of an eluate faster when peaks other than that of an object to be analyzed appear. ;CONSTITUTION: At the time of using the HPLC analyzing method, the flow velocity of an eluate is made faster as compared with the flow velocity of the eluate used when the peak of an object to be analyzed appears when peaks other than that of the object appear during the analysis of the same sample. Therefore, the influence of the leadings or tailings of unnecessary peaks other than that of the object on main peaks can be eliminated and high-precision analysis can be conducted. In addition, the measuring time can be shortened. When an HPLC device connected with a microcomputer, etc., is used for automatically changing the flow velocity of the eluate in accordance with a preset condition, the analysis can be automated. In addition, in case the object to be analyzed is a substance having enzyme activity and the enzyme activity is utilized for detecting the peak of the object, it is preferable to connect the HPLC device to a post-column reactor.;COPYRIGHT: (C)1994,JPO&Japio
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