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Method for measuring analytes and its use for measuring anti-TSH receptor autoantibodies in patient serum
Method for measuring analytes and its use for measuring anti-TSH receptor autoantibodies in patient serum
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机译:测量分析物的方法及其在患者血清中测量抗TSH受体自身抗体的用途
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PCT No. PCT/EP94/02748 Sec. 371 Date Jul. 9, 1996 Sec. 102(e) Date Jul. 9, 1996 PCT Filed Aug. 18, 1994 PCT Pub. No. WO95/06258 PCT Pub. Date Mar. 2, 1995Method for the determination of an analyte in a volume of a fluid sample, in particular of anti-TSH receptor autoantibodies in a patient serum, in which one or more determination reagent(s) which contains or which contain a) a predetermined amount of a binder (B) for the analyte (A) and b) a predetermined amount of a competitor (K) which is likewise bound by the binder (B), in such a way that the extent of its binding to the binder (B) is correlated with the simultaneous presence of the analyte (A) and/or its amount in the sample, the competitor (K) being labelled, or being capable of being labelled, with a label which permits its qualitative and/or quantitative determination, is or are added to the sample with the formation of a liquid reaction mixture, wherein c) the liquid reaction mixture is reacted simultaneously or subsequently with a solid phase to which a substance (Imm) for the selective immobilization of the amount of competitor (K) not bound to the binder (B) is bound, and optionally with a labelled reactant (Label) for the immobilized competitor (K), and, after a quantitative separation of the solid phase from the liquid reaction mixture, the amount of the competitor (K) bound to the solid phase is determined in a manner known per se by means of physical and/or chemical detection of the label bound to said competitor, and the determined amount of said competitor (K) is correlated with the presence and/or amount of the analyte (A) in the original sample.
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