Give the chemiluminescence where was reinforced from the chemiluminescence chemical compound which uses two value cationic surface active agents manner and the constituent

摘要

A dicationic surfactant for enhancing the chemiluminescence of chemiluminescent compounds particularly dioxetanes which are triggerable is described. The surfactant has the formulaPPX.sup.- (R. sub.1).sub.3 A.sup.+ CH.sub.2 --Link--CH.sub.2 A.sup.+ (R.sub.2).sub.3 X. sup.-PPwherein A is selected from the group consisting of phosphorus and nitrogen atoms separately or together, wherein X is an anionic counterion and R.sub.1 and R.sub.2 are selected from the group consisting of unsubstituted and substituted alkyl and aralkyl groups containing 1 to 20 carbon atoms and R.sub.1 and R.sub.2 can be the same or different, and wherein Link is a carbon chain group selected from the group consisting of dialkylenearyl, aryl, alkylene, alkenylene and alkynylene groups containing 4 to 20 carbon atoms. The surfactants are particularly useful in Western and Southern blot assays.