Streptococcal heat shock protein family members hsp7270, DNA Sequence encoding such polypeptides, expression vector, a recombinant DNA Molecule.Method for producing a polypeptide Antibody or fragment thereof, or a fragment thereof and monoclonal antibody

摘要

New heat shock proteins (HSPs), s.hypodermis and s.agalactia, with molecular weight of 70-72 KVA, are immune related peptides derived from the nucleic acid and amino acid sequences of s.pnemoniae HSP72 (secid ns: 4, SEC NID ns: 5),S. Nucleotide and amino acid sequences of pyogenes HSP70 (SEC ID ns: 19; sec ID ns: 20)Denucleyt and amino acid sequence extracted from HSP70 of S. agalactiae (section ID ns: 21; section ID ns: 22);Antibodies, expression vectors and recombinant hosts combined with HSPs and recombinant DNA methods are used to produce polypeptides related to HSPs and immunity. Polypeptides, DNA sequencing and antibodies of the invention provide new means for the diagnosis, prevention and / or treatment of diseases caused by Streptococcus. It describes a new method for producing antibodies against HSP72 and immune related peptides. These peptides are used to develop vaccines and methods to prevent s.pnemone infection. Last,This report also introduces the dechlorination method of S. pnemoniae in biological samples, using DNA detector of HSP72 bacterial polypeptide and polypeptide, immune endocrine, antibody or nucleic acid encoded by HSP72 polypeptide.