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Microorganism of the genus zymomonas mobilis containing one or more exogenous vector genes and the respective process for producing ethanol

机译:含有一种或多种外源载体基因的运动发酵单胞菌属微生物及其生产乙醇的方法

摘要

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to fermen pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L- ribulokinase, and L--ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
机译:本发明涉及通常不发酵戊糖并且经遗传转化为戊糖戊糖以产生乙醇的微生物,以及利用该微生物的发酵方法。实例包括运动发酵单胞菌(Zymomonas mobilis),其已被大肠杆菌基因的组合转化为木糖异构酶,木酮糖激酶,转醛醇酶,转酮醇酶,L-阿拉伯糖异构酶,L-核糖激酶和L-核糖5-磷酸4-表异构酶。添加的基因的表达在运动发酵单胞菌启动子的控制下。这些新产生的微生物可用于发酵由半纤维素和纤维素水解产生的戊糖和葡萄糖,以产生乙醇。

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