Procedure for obtaining, spreading and applying Massively Trichoderma sp antagonist in integrated Biological Control of Fusarium oxysporum dianthi and other fungi fitopa - togenos Soil

摘要

The present Invention relates to a method for Trichoderma sp Massively with the object of biological agent use as antagonist for Preventing and limiting DevelopmentAnd Fusarium oxysporum dianthi in crops of Flowers, especially of Carnation, comprising the steps of: A.Trichoderma strains obtained from various sources of soil or water through the separation of Microorganisms through the technique of dilution and then PURIFYING through monospu00f3rico cropsS on PDA Culture medium to remove any microbial contamination that may contain in its original State. B.Mother maintain the collection of strains that have been selected for their potential as agents of Biological antagonism against Fusarium oxysporum dianthi,This through maintaining Crop strains Daughters and to avoid Manipulation of the parent Strain.As well as the Renewal of virulence of the parent Strain every six months by Planting in barren soil with pieces of Carnation Cuttings also sterile.In order that the fungus Active foraging capacity. C.A procedure for the assessment of Potential Biological antagonistic Trichoderma, using the methodology of antagonism in vitro on PDA and sterile soil.Based on the Measurement of the rate of growth of Trichoderma and a visual method to quantify the sporulation of Trichoderma on FusariumBecause these are the two main features that we consider to catalog A Strain as appropriate to antagonism against Fusarium oxysporum.A procedure to propagate the Trichoderma sp obtained according to the present Invention, which includes the steps of.Disinfestation of barley Dry to remove fungal contaminants B. soften the Barley in water in order to achieve the optimal Texture for the development of the fungus on the grain. C.Drain the excess Water to stop the Softening process. D.Pack the Barley in high density plastic bags and Seal them before their Steam sterilization, to avoid the direct handling of the grain,Using suitable bags withstand Steam and gas exchange after sufficient for the good Development of the Fungus. E. Steam sterilized bags. ...