High density fermentation produces recombinant protein E. Coliform cell

摘要

The present invention discloses a kind of high density fermentation production foreign proteins in E. Coliform cell, which is to carry out batch and fed-batch step by transformation plasmid carrying inducible promoter, without usually limiting in cell growth factor substrate or metabolic by-product albumen exprimed is separated and purified from culture medium, thus the concentration of substrate adjusts and/or controls by the analysis of automanual system and ingredient in fed-batch phase, wherein being to maintain carbon source concentration on average is 0.1 grams per liter but maintains cell (micron)=micronimax of indeterminate growth to 25 grams per liters, production foreign protein starts in the fed batch phase, inducing the product synthesized in available nitrogen, phosphorus and the continuous feed of salt microelement after cell concentration range is induced for 20 to 80 grams per liters by promoter, wherein pOi2 values adjust the fed batch phase that the oxygen for passing through and supplying accordingly enters the entire period in 5 to 25 range of suspension of fermenting. It is required that being also E. Bacillus expression vector is suitable for the E of high-cell density and conversion under the fermentation condition for expressing foreign protein. Does coliform express host RV308 (pHKK), by converting RV308 (ATCC 31? 608) with specific pHKK vectors.