Method for searching substances with modulating effect on an interleukin-5 receptor-dependent cellular signaling site

摘要

PCT No. PCT/EP94/01735 Sec. 371 Date May 29, 1996 Sec. 102(e) Date May 29, 1996 PCT Filed May 27, 1994 PCT Pub. No. WO94/28170 PCT Pub. Date Dec. 8, 1994A process is disclosed for screening substances having a modulating effect on the interleukine-5-receptor-dependent signal tranmission path in mammal cells. Test substances are applied on cells which express the functional interleukine-5-receptor and are transformed with a recombinant DNA containing a reporter gene and a regulatory sequence functionally linked thereto which is sensitive to a change in concentration of a secondary messenger substance of the interleukine-5-receptor-dependent signal transmission path. Substances specific to the interleukine-5-receptor-dependent signal transmission path and with potential pharmacological effect may be identified by this process.