Method for the at least partially sterile preparation, inoculation with the mycelium and in the sterile storage units conveying a nutrient medium, and an apparatus for carrying out said method.

摘要

The process and apparatus according to the invention are suitable for sterile and/or partially sterile preparation, inoculation and bagging of mushroom substratum. The joint application of the process and apparatus enables the use of agricultural wastes and the production of substratum in reduced time so that the yield can be multipled compared with the practices known so far and the expenditures are lower than those known so far. In the course of the process according to the invention dry straw and/or corn-cob are comminuted to the required size and admitted together with additive into a closed system. The material is treated with 90 DEG C. hot water until 70% moisture content is reached in the first step, while it is continuously mixed, then it is sterilized by heating for 35 minutes. Next the material is cooled to 25 DEG C. and the internal pressure of the apparatus is equalized to atmospheric pressure. This is followed by inoculation of the basic material from partially sterile chamber with inoculum of 1.5 mm grain size. After mixing for 5 minutes the so-prepared material is bagged in partially sterile space for the purpose of interweaving of cultivation, then it is sealed. The apparatus according to the invention is formed with a double walled cylindrical jacket of narrowing extension provided with lid, hermetically sealed and mixing worm is arranged in its centreline. The apparatus is provided with inlet and outlet valves and pipe system for the basic material, inoculum, steam water and air. During operation of the apparatus the mixing of the material is solved with bi-directional mixing while it is being sterilized and the inoculum ground. The process and apparatus are capable to produce mushroom varieties of higher yield and more sought-after on the market and at a more favorable cost than those produced with the practice and efficiency known so far.