RECOMBINANT VIRUS, ITS PRODUCTION BY LIPOSOME MEDIATED COTRANSFECTION AND COMPOSITIONS AND METHODS INVOLVING P53 ADENOVIRUS CONSTRUCTS

摘要

Described are simplified and efficient methods for preparing recombinant adenovirus using liposome-mediated cotransfection and the direct observation of a cytopathic effect (CPE) in the transfected cells. Also disclosed are compositions and methods involving novel p53 adenovirus constructs, including methods for restoring p53 function and tumor suppression in cells and animals having abnormal p53.