首页>
外国专利>
Analysis of expression of rat cytochrome p450 isoenzymes and phase ii conjugating enzymes
Analysis of expression of rat cytochrome p450 isoenzymes and phase ii conjugating enzymes
展开▼
机译:大鼠细胞色素p450同工酶和II期结合酶的表达分析
展开▼
页面导航
摘要
著录项
相似文献
摘要
Hepatocyte culturing system, primer sets and an analytical method for selectively detecting and quantitatively assessing the levels of mRNA expression of the major isoenzymes of cytochrome P450 (CYP450 1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2 and 4A1), fatty acyl-CoA oxidase (FACO) and select Phase II conjugating enzymes (UDPGT, GST and ST) in the rat using specific 5' and 3' oligonucleotide primers and reverse transcriptase-polymerase chain reaction. The method closely reproduces the expression obtained from rat liver tissue following treatment with the same enzyme inducers. Constitutive and inducible expression was maintained by resuspending, culturing and then overlaying adult rat hepatocytes with an extracellular matrix such as Matrigel(R).
展开▼