Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry

摘要

The invention provides an analytical method for determining the nucleotide sequence of nucleic acid analytes, including chemically modified oligonucleotides. This new method utilizes matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) with delayed pulsed ion extraction.