1-aminocyclopropane-1-carboxylate synthase genes from (pelargonium) and (rosa) to control ethylene levels in geraniums and roses

摘要

A gene which encodes an ACC synthase is isolated from rose plants, specifically Rosa (cardinal red). This gene is modified for expression in transgenic plants. Isolation of high quality mRNA for gene isolation is achieved through use and adaptation of a 2-butoxyethanol precipitation technique using large amount of initial tissue in order to achieve critical mass for precipitation.