AUTOMATIC SEQUENCER/GENOTYPER HAVING EXTENDED SPECTRAL RESPONSE

摘要

An advanced imaging spectrograph system (10) includes an electrophoresis device (12), an optical imaging device (14), and a processor (16), configured to provide for slab-gel DNA sequencing and genotyping with high throughput sequencing. The system is based on the integration of improved electrophoresis structures with an imaging spectrophotometer that records the entire emission spectra along an imaging line across a sequencing gel (or capillary array). In one embodiment, the electrophoresis device (12) includes a large number of parallel lanes (18) formed in an electrophoresis gel (20) which is sandwiched between two flat rectangular glass plates (22). The system includes spectral shape matching to improve dye identification allowing the use of dyes having nearly any emission spectra and allowing greater than four dye multiplexing.