SIMULTANEOUS HUMAN ABO AND Rh(D) BLOOD TYPING OR ANTIBODY SCREENING BY FLOW CYTOMETRY

摘要

Flow cytometric methodology is provided for simultaneous determination of (1) ABO and Rh(D) typing of human red cells, (2) natural isoantibodies in plasma, and (3) screening for alloantibodies in plasma. The method includes (a) the use of a unique combination of fluorescent labeled antibodies to A, B and Rh(D) antigens to carry out (1); (b) different sized beads coated with blood group substances A and B to carry out (2); and (c) the differential fluorescent labeling of screening reagent red blood cells for flow cytometric analyses to carry out (3). The routine ABO and Rh(D) typing and antibody screening of human blood for both isoantibodies and alloantibodies can be determined in three individual reactions compared to 7 to 10 tests currently performed in blood banks.