The present invention relates to a method for producing a physiologically active substance by constructing a mammary gland-specific expression system using the promoter region of the betacase gene of Korean native goat. More specifically, the expression regulatory region of beta-casein gene, which is specifically expressed in mammary gland tissue, is isolated and recombined with a biologically active substance gene and a transcription termination regulatory region to produce a physiologically active substance in the mammalian body. And new recombinant vectors pGbc, p Gbc-L and pGbc-S (1997.3.3 KCTC 8790P, 8791P, 8792P). PGbc_S, pGbc_L, and pGbc, which were prepared by using the promoter of the betacase gene of Korean native goat, were used to express human granulocyte colony stimulating factor (human Granulocyte Colony Stimulating Factor) and human Granulocyte Macrophage Colony Stimulating Factor) was expressed from milk of HC11 cells and transgenic mice derived from mouse mammary gland tissue. As a result of the invention of the mammary gland-specific expression system, the problem of the maintenance of the activity of the physiologically active substance, the high production unit price, the scale-up, and the stability are solved.
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