Method for preparing high purity crystalline NOCC and method for controlling molecular weight of water soluble chitosan using enzyme

摘要

purpose;An object of the present invention is to provide a method for preparing high purity crystalline NOCC and a method for controlling molecular weight of NOCC.;Configuration;After dispersing chitosan in isopropanol and warming it to about 30 ° C, NaOH aqueous solution was added dropwise, and after stirring, monochloroacetic acid dissolved in isopropyl alcohol was added, and the white powder in this solution became transparent, and then it was kept at room temperature. After stirring for a certain period of time, the pH was adjusted to 7, the residue was filtered, washed with a mixture of methanol and water, dissolved in methanol, stirred, washed with methanol, filtered, and dried to prepare high-purity crystalline NOCC, and NOCC Warm the aqueous solution to 50 ~ 60 ℃, add cellulase or chitosanase, adjust the pH to 6 ~ 7 after enzymatic digestion, and then concentrate and dry the filtrate to adjust the molecular weight.