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METHOD OF DETERMINATION OF GLYCOSYLATED HEMOGLOBIN IN THE PRESENCE OF NONGLYCOSYLATED HEMOGLOBIN, ANALYTICAL TEST KIT
METHOD OF DETERMINATION OF GLYCOSYLATED HEMOGLOBIN IN THE PRESENCE OF NONGLYCOSYLATED HEMOGLOBIN, ANALYTICAL TEST KIT
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机译:分析试剂盒中非糖基化血红蛋白存在下糖基化血红蛋白的测定方法
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摘要
FIELD: analytical biochemistry, medicine. SUBSTANCE: method involves the following stages: optional hemolysis of sample to release cell-bound hemoglobin; contact of indicated sample or hemoglobin isolated from indicated sample according to the stage described below using signal-forming molecules and involving conjugate consisting of one or more dihydroxybornyl residues or their salts bound with signal-forming label; isolation of glycosylated and nonglycosylated hemoglobin and any bound with it molecules from the sample or reaction mixture and stage described above evaluation of indicated signal-forming molecules bound with isolated hemoglobin and/or any nonhemoglobin-bound signal-forming molecules. Invention relates to analytical kit designated for the use according to method proposed. New method of analysis can be used for diagnosis and monitoring of diabetes mellitus especially. EFFECT: improved method of assay. 26 cl, 3 dwg, 1 tbl
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