METHOD OF PREPARING MICROBE PESTICIDE WITH BIPOLAR COVER, MICROBE PESTICIDE WITH BIOPOLYMERIC COVER, METHOD OF PREPARING MICROBE PESTICIDE OF BUOYANT TYPE

摘要

FIELD: microbiology, biotechnology, pesticides. SUBSTANCE: invention relates to preparing agents for plant protection, in part, pesticides with biopolymeric cover. Method involves mixing biopolymer with water, heating a mixture to 80-121 C up to gel or paste formation, cooling to the temperature from room point to 60 C and mixing with one of the following strain of microorganisms: Bacillus subtilis krietiensis ATCC 55078 or 55079, Pseudomonas fluorescens ATCC 27663, Gliocladium virens ATCC 52045, Trichoderma reesei ATCC 28217, Trichoderma harzianum ATCC 52445, Trichoderma hamatum ATCC 52198, Trichoderma viride ATCC 52440, Streptomyces cacaoi asoensis ATCC 19093, Bacillus thuringiensis ATCC 13367, Beauveria bassiana ATCC 26851, 48585 or 48023, Hirsutella thomsonii ATCC 24874, Metarhizium flavoviride ATCC 32969, Verticillium lencanii ATCC 46578, Collectotrichum gloeosporioides f. sp. jussiaeas ATCC 52634 taken in the amount 10⁴-10¹⁴ cells/1 g pesticide. Obtained mixture is formed and dried and pesticide is prepared. Before mixing the cooled gel-like or paste-like biopolymer with microorganism or in the process of their mixing stabilizing agent and nutrient addition are added expediently. The following substances can be used as a biopolymer (one component): grains, tubers, root crops,, starch extracted from their, gelrit, natural wax and resins and their derivatives. Stabilizing agents: glycerol, milk, skim milk, dry milk, vegetable oil, animal fat. Nutritional addition: soybean meal, cotton seed meal, skim milk, peptones from animal or vegetable raw, milk, powder-like chitin and compost, wheat bran, yeast and corn extracts, molasses, glucose, sucrose, dextrin, iron, manganese, zinc, cobalt, soil, soil extract. For preparing buoyant type pesticide the above described obtained pesticide is treated with the following material exhibiting buoyancy: swollen grains, rice bran, nut shells, barley bran, corn cob covering leaves, barley straw, corn stems, millet stems, wood saw-dust, rice or wheat straw. Obtained pesticides affect more insect species. EFFECT: improved method of preparing, enhanced effectiveness of pesticide. 4 cl, 10 tbl